MILK COMPOSITION & ITS CONSTITUENTS : NUTRITIONAL IMPORTANCE OF MILK

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MILK COMPOSITION & ITS CONSTITUENTS – NUTRITIONAL IMPORTANCE OF MILK

Milk is nature’s ideal food for infants and  growing  children.  The importance of milk in our diet has been recognized since Vedic times, and all modern research has only supported and reinforced this view. In fact, milk  is  now  considered  not  only  desirable  but  essential  from the time the child is born. The baby is recommended to be breast-fed until it is weaned and thereafter  given  cow/buffalo/goat  milk  till  he  or she reaches 12 years of age.

Milk is almost an ideal food. It has high nutritive value. It supplies body-building proteins, bone-forming minerals and health-giving vitamins and furnishes energy-giving lactose and milk fat.  Besides  supplying certain essential fatty acids, it contains the above nutrients in an easily digestible and assimilable form. All these properties make milk an important food for pregnant mothers, growing children,  adolescents, adults, invalids, convalescents and patients alike.

Factors affecting milk composition:

 Genetic

Breed and individual cow: Milk composition varies considerably among breeds of dairy cattle: Jersey and Guernsey breeds give milk of higher fat and protein content than Shorthorns and Friesians. Zebu cows can give milk containing up to 7% fat.

Variability among cows within a breed: The potential fat content of milk from an individual cow is determined genetically, as are protein  and lactose levels. Thus, selective breeding can be used to upgrade milk quality. Heredity also determines the potential milk production of the animal. However, environment and various physiological factors greatly influence the amount and composition of milk that is actually produced. Herd recording of total milk yields and fat and SNF percentages will indicate the most productive cows, and  replacement  stock  should  be bred from these.

Environmental

Interval between milkings: The fat content of milk varies considerably between the morning and evening milking because there is  usually  a much shorter interval between the morning and evening milking than between the evening and  morning milking. If cows were  milked at  12- hour intervals the variation in fat content between milkings would be negligible, but this is not practicable on most farms.  Normally,  SNF content varies little even if the intervals between milking vary.

Stage of lactation: The fat, lactose and protein contents of milk vary according to stage of lactation. Solids-not-fat content is usually highest during the first 2 to 3 weeks, after  which  it  decreases  slightly.  Fat content is high immediately after calving but soon begins to fall, and continues to do so for 10  to  12  weeks,  after  which  it  tends  to  rise again until the end of the lactation.

Age: As cows grow older the fat  content  of  their  milk  decreases  by about 0.02 percentage units per lactation. The fall  in  SNF  content  is much greater.

Feeding regime: Underfeeding reduces both  the  fat  and  the  SNF content of milk produced, although SNF content is more sensitive to feeding level than fat content. Fat content and fat composition are influenced more by roughage (fibre) intake.

The SNF content can  fall  if  the  cow  is  fed  a  low-energy  diet,  but  is not greatly influenced by protein deficiency, unless  the  deficiency  is acute.

Disease: Both fat and SNF contents can be reduced by disease, particularly mastitis.

Completeness of milking: The first milk drawn from the udder is low in fat while the last milk (or strippings) is always quite high in fat. Thus it is essential to mix thoroughly all the milk removed, before taking a sample for analysis.  The  fat  left  in  the  udder  at  the  end  of  a  milking is usually picked up  during  subsequent  milkings,  so  there  is  no  net loss of fat.

Physical and chemical properties of milk

 Milk is an emulsion  or  colloid  of  butterfat  globules  within  a  water- based fluid that contains  dissolved  carbohydrates  and  protein aggregates with minerals. Because it is produced as a food source for the young, all of its contents  provide  benefits  for  its  growth.  The principal requirements are energy (lipids, lactose, and protein), biosynthesis of non-essential  amino  acids  supplied  by  proteins (essential amino acids  and  amino  groups),  essential  fatty  acids, vitamins and inorganic elements, and water.  Butterfat  is  a  triglyceride (fat) formed from fatty acids such as mystiric, palmitic, andoliec acid.

Lipids: Initially milk fat is secreted in the  form  of  a  fat  globule surrounded by a membrane. Each fat  globule  is  composed  almost entirely of triacylglycerols and is surrounded by a membrane consisting of complex lipids such as phospholipids, along with proteins. These act as emulsifiers which keep the individual globules from coalescing and protect the contents  of  these  globules  from  various  enzymes  in  the fluid portion of the milk. Although 97–98% of lipids  are  triacylglycrols, small amounts of di- and monoacylglycerols, free cholesterol and cholesterol esters, free fatty acids, and phospholipids are also present. Unlike protein and carbohydrates, fat composition in milk varies widely in the composition due to genetic, lactational, and nutritional factor difference between different species.

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Like composition, fat globules vary in size from less than 0.2 to about 15 micrometers in diameter between different  species.  Diameter  may also vary between animals  within  a  species  and  at  different  times within a milking of a single animal. In unhomogenized cow’s milk, the fat  globules have an average diameter of two to four  micrometers  and with homogenization, average around 0.4 micrometers. The fat soluble vitamins  A,  D,  E,  and  K  along  with  essential  fatty  acids  such  as linoleic and linolenic acid are found within  the  milk  fat  portion  of  the milk.

Proteins: Normal bovine milk contains 30–35 grams of protein per liter of which about 80% is arranged in casein micelles.

The largest structures in the  fluid  portion  of  the  milk  are  ‘casein micelles’ aggregates of several thousand protein  molecules  with superficial resemblance to surfactant micelles, bonded with the help of nanometer-scale particles of calcium phosphate. Each casein micelle is roughly spherical and about a tenth of a micrometer across. There are four different types of casein proteins: αs1-, αs2-, β-, and κ-caseins. Collectively, they make up around 76–86% of the protein in milk,  by weight. Most of the casein proteins are bound into the micelles. There are several competing theories regarding the precise structure of the micelles, but they share one important feature: the outermost  layer consists of strands of one type of protein, k-casein, reaching out from the body of the micelle into the surrounding fluid. These kappa-casein molecules all have a  negative  electrical  charge  and  therefore  repel each other, keeping  the  micelles  separated  under  normal  conditions and in a stable colloidal  suspension  in  the  water-based  surrounding fluid.

Milk contains dozens of other types of proteins beside the  caseins including enzymes. These other proteins  are  more  water-soluble  than the caseins and do not form larger structures. Because  the  proteins remain suspended in the whey left behind when the caseins coagulate into curds, they  are  collectively  known  as  whey proteins.  Whey proteins make up approximately 20% of the protein in milk, by weight. Lactoglobulin is the most  common  whey  protein  by  a  large  margin while lactoalbumin is also present in meager amounts.

Carbohydrates: Milk contains several different Carbohydrates including lactose, glucose, galactose, and other  oligosaccharides.  The  lactose gives milk its sweet taste and contributes approximately 40% of whole cow’s milk’s calories.  Lactose  is  a  disaccharide  composite  of  two simple sugars, glucose and galactose. Bovine milk averages 4.8% anhydrous lactose, which amounts to about 50% of the total solids of skimmed milk. Levels of lactose are dependent upon the type of milk as other carbohydrates can be present at higher concentrations that lactose in milks.

Salts, minerals, and vitamins: Minerals or milk salts are  traditional names for a variety of cations and anions within bovine milk. Calcium, phosphate, magnesium, sodium, potassium, citrate, and chlorine are all included as minerals and they typically  occur  at  concentration  of  5– 40%. The milk salts strongly interact with casein, most notably calcium phosphate. It is present in excess and often, much greater excess of solubility of solid calcium phosphate In addition  to  calcium,  milk  is  a good source of many other vitamins. Vitamins A, B6, B12, C, D, K, E, thiamine, niacin, biotin, riboflavin, folates, and pantothenic acid are all present in milk.

Nutritional Components in Milk

Energy

 The energy in milk  comes  from  its  protein,  carbohydrate  and  fat content, with the exception of skim milk that has virtually no fat. The calorie/energy content of milk varies depending on the fat content. For example, whole standardized milk (3.5% milk fat) provides 68kcals per 100ml; semi-skimmed milk (1.7% milk fat) provides 47kcals/100ml and skimmed milk (0.3% milk fat) provides 35kcals /100ml.

Water

 Milk is approximately 87%  water,  so  it  is  a  good  source  of  water  in the diet. The water  content  of  milk  varies  from  one  type  to  another type of milk, animal and their species.

Carbohydrate

 Milk is approximately  4.9%  carbohydrate  in  the  form  of  lactose. Lactose is a disaccharide made up of glucose and galactose bonded together. Before it can be used by the body, the bond must be broken by the enzyme lactase in the  small  intestine.  People  that  have decreased activity of lactase in the small intestine may have problems digesting lactose and this is referred to as lactose intolerance or malabsorption. There is approximately 9.7g of lactose in 1 glass/200ml of semi skimmed milk.

Fat

Milk is approximately 3.4% fat. The fatty acids in milk  fat  are approximately 65% saturated, 29% monounsaturated, and 6% polyunsaturated. The polyunsaturated fatty  acids  in  milk  fat  include small amounts of the essential fatty acids linoleic and linolenic, and approximately 5% trans  fatty  acids.  An  important  trans  fatty  acid  in milk fat  is  conjugated  linoleic  acid  (CLA,  18:2).  There  are  several types (isomers) of CLA in milk that have been shown to inhibit cancer and help maintain lean  body  mass  while  promoting  the  loss  of  body fat. Cholesterol is associated  with  fat  so  the  content  will  vary depending on the fat content of the dairy product. One glass of 2% fat milk contains around 8% of the Daily Reference Intake (DRI) for cholesterol.

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Protein

Milk is a source of “high biological value” protein – which means that it provides us with all the essential  amino  acids  that  the  body  cannot make itself. Milk contains approximately 3.5% protein by weight which can be divided into two main groups: caseins and whey proteins. Approximately 80% of the protein in milk is casein based and 20% is whey based. Casein is the predominant protein in  milk  and  can  be divided into four major types: alpha, beta, gamma and kappa caseins.

Whey protein comprises the rest of the milk protein and is composed predominantly of beta -lactoglobulin and alpha-lactalbumin.  But  other whey proteins include serum albumin, immunoglobulins (IgA, IgG, IgM), protease peptones, lactoferrin and transferrin. Whey proteins have been proposed  to  have some benefits to  athletes for  muscle recovery and for preventing mental fatigue.

Fat soluble vitamins

 Whole milk contains some vitamin A,  however levels  in semi  skimmed and skimmed milks are much lower. This is because vitamin  A  is dissolved within the milk fat fraction which is removed to varying levels when producing lower fat milk products. Whole milk  contains approximately 62µg of vitamin  A  per  glass  (200ml)  which  provides about 9% of an adult’s daily vitamin A requirement.

Vitamin D plays an important role in the absorption of calcium and phosphorus and is essential for healthy bones and teeth.  There is very little vitamin D in  milk  and  in  fact  only  trace  amounts  so  processed milk is fortified with vitamin D.

Vitamin E is naturally found in low levels within milk, a 200ml serving of semi-skimmed milk typically provides 0.04mg of vitamin E.

Vitamin K is essential for correct blood clotting. There is  little  or  no vitamin K  naturally  found  in  milk  although  small  amounts  may  be found in cheese.

Water soluble vitamins

 Milk contains appreciable amounts of many of the B vitamins, and is particularly rich in vitamin B12. It is predominantly  found  in  foods  of animal origin and therefore milk and dairy products are excellent sources. 1 glass/200ml of semi skimmed milk will provide an adult (19-50 years) with the full daily  requirement  for  vitamin  B12.  Exclusion  of milk and dairy products from the diet can therefore significantly reduce vitamin B12 intakes. This is of  particular  concern  when  all  animal products are excluded from the  diet –  as  with  the  vegan  diet  –  which can lead to  vitamin  B12  deficiency  if  suitable  alternatives  or supplements are not taken.

Thiamin (vitamin B1)  and  riboflavin  (vitamin  B2)  are  also  present  in milk. 1 glass/200ml of semi skimmed milk will provide an adult (19-50 years) with 15% of their daily requirement for thiamin. Similarly 200ml glass of  semi-skimmed milk provides 45% of an adult’s (19-50  years) daily requirement for riboflavin.

Milk provides an individual with up to 3% of the daily  requirement for niacin. A 200ml  glass  of  semi-skimmed  milk  provides  a  6  year  old child with 18.6% of  his  or  her  daily  folate  requirement  and  an  adult with 9.3%.

Pyridoxine (Vitamin B6) is an essential vitamin involved in protein metabolism and is required for the formation of red blood cells and for maintaining a  healthy  immune  and  nervous  system.  This  vitamin  is only present in small amounts in milk.

Vitamin C is required  for  the  correct  structure  and  maintenance  of blood vessels, cartilage, muscle and bone. A glass/200ml of semi-skimmed milk will provide a child with 14% of the daily requirement for vitamin C and an adult up to 10.3%.

Minerals and trace elements

 Milk is a good source of calcium, which is  essential  for  the  healthy growth and maintenance of teeth and bones and is a vital function in blood clotting and muscle contraction.  A 200ml glass of semi-skimmed milk can provide a 6-year-old child with over half (55%) of his or her calcium requirement and can provide an adult (19-64years) with over a third (35%) of his or her daily calcium requirement.

Phosphorus is also essential  for  healthy  bones  and  teeth  as  well  as cell membrane structure, tissue growth  and  regulation  of  pH  levels  in the body.   A glass/200ml of semi skimmed milk will provide a child of 6 years with 55% of  their  daily  requirement  for  phosphorus  and  an adult  (19-50 years)  with 36%.1 glass (200ml) of semi-skimmed milk will provide a child of 6 years with 96% of their daily  requirement  for  iodine  and  an  adult  (19-50 years) with 44%. (These figures are for winter milk, which may contain slightly higher levels of iodine than summer milk.)

Magnesium is abundant in bone and in all cells in the body. 1 glass/200 ml of semi skimmed milk will provide a child of 6 years with 19% of their daily requirement for  magnesium  and  an  adult  (19-50  years) with 7.5%.

Zinc is a constituent of many enzymes in the body; its role is to fight infections, growth development, for sexual development, wound healing and for our sense of taste. 1 glass/ 200 ml of semi skimmed milk will provide a child of  6  years  with  12.3%  of  their  daily  requirement  for zinc and an adult (19-50 years) with 11%.

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Potassium is mainly present in the fluid of the cells in the body and is important for fluid  balance,  muscle  contraction,  nerve  conduction  as well as for  the  correct  functioning  of  the  heart.  1  glass/  200  ml  of semi skimmed milk will provide a  child  of  6  years  with  29%  of  their daily requirement for potassium and an adult (19-50 years) with 9%.

Other minerals

 Sodium, selenium and iron are also found in milk in low levels.

Minor Biological Proteins and Enzymes

 Other minor proteins and enzymes in milk that are of nutritional interest include lactoferrin and lactoperoxidase. Lactoferrin is an iron binding protein that plays a role  in  iron  absorption  and  immune response. The use of lactoferrin as an antimicrobial agent is also very important.

Lactoperoxidase is an enzyme that, in  the  presence  of  hydrogen peroxide and thiocyanate,  has  antibacterial  properties.  Lipase  is  a group of enzymes that break down fats, are present in milk but are inactivated by pasteurization, which increases the shelf life of milk. Apopular belief among raw milk consumers  is  that  the  native  lipase  in milk plays an important role in the digestion of fat. Lactase (ß- galactosidase) is the enzyme responsible for the  breakdown  of lactose into glucose and galactose for digestion.

Table 2. Nutrient content of milk varieties by 100 g reference amount. Compiled from the USDA Nutrient Data

    Cow      
Component Uni t Whol e (3.25

% fat)

Reduce d Fat (2%

fat)1

Lowfa t   (1%

fat)1

Skim

1

Goat Shee p Water Buffal o
Overall Composition       (Amount per 100 g   )
Water g 88.32 89.33 89.92 90.8

4

87.0

3

80.70 83.39
Energy kca

l

60 50 42 34 69 108 97
Carbohydrat

e (Lactose)

g 4.52 4.68 4.99 4.96 4.45 5.36 5.18
Fat g 3.25 1.97 0.97 0.08 4.14 7.00 6.89
Protein g 3.22 3.30 3.37 3.37 3.56 5.98 3.75
Minerals

(Ash)

g 0.69 0.71 0.75 0.75 0.82 0.96 0.79
Vitamins
Vitamin A µg 28 55 58 61 57 44 53

 

Thiamin (Vitamin

B1)

mg 0.044 0.039 0.02 0.04

5

0.04

8

0.065 0.052
Riboflavin

(Vitamin B2)

mg 0.183 0.185 0.185 0.18

2

0.13

8

0.355 0.135
Niacin (Vitamin

B3)

mg 0.107 0.092 0.093 0.09

4

0.27

7

0.417 0.091
Pantothenic Acid (Vitamin

B5)

mg 0.362 0.356 0.361 0.35

7

0.31

0

0.407 0.192
Vitamin    B6

(Pyridoxine)

mg 0.036 0.038 0.037 0.03

7

0.04

6

0.060 0.023
Vitamin B12

(Cobalamin)

µg 0.44 0.46 0.44 0.53 0.07 0.71 0.36
Vitamin C mg 0.0 0.2 0.0 0.0 1.3 4.2 2.3
Vitamin D IU 40 43 52 41 12 ND3 ND
Vitamin E mg 0.06 0.03 0.01 0.01 0.07 ND ND
Folate µg 5 5 5 5 1 7 6
Vitamin K µg 0.2 0.2 0.1 0.0 0.3 ND ND
Minerals (Ash)
Calcium mg 113 117 119 125 134 193 169
Copper mg 0.011 0.012 0.010 0.01

3

0.04

6

0.046 0.046

 

Iron mg 0.03 0.03 0.03 0.03 0.05 0.10 0.12
Magnesium mg 10 11 11 11 14 18 31
Manganese mg 0.003 0.003 0.003 0.00

3

0.01

8

0.018 0.018
Phosphorus mg 91 94 95 101 111 158 117
Potassium mg 143 150 150 156 204 137 178
Selenium µg 3.7 2.5 3.3 3.1 1.4 1.7 ND
Sodium mg 40 41 44 42 50 44 52
Zinc mg 0.40 0.43 0.42 0.42 0.30 0.54 0.22

Composition of milk obtained from different mammals

SPECIES FAT

%

PROTEIN

%

LACTOSE

%

ASH

%

TOTAL SOLIDS

%

Buffalo, 10.4 5.9 4.3 0.8 21.5
Camel 4.9 3.7 5.1 0.7 14.4
Cow: Ayrshire Brown Swiss Guernsey Holstein Jersey

Zebu

 

4.1

4.0

5.0

3.5

5.5

4.9

 

3.6

3.6

3.8

3.1

3.9

3.9

 

4.7

5.0

4.9

4.9

4.9

5.1

 

0.7

0.7

0.7

0.7

0.7

0.8

 

13.1

13.3

14.4

12.2

15.0

14.7

Goat 3.5 3.1 4.6 0.79 12

 

Human 4.5 1.1 6.8 0.2 12.6

Table is adapted from course notes by Robert  D.  Bremel, University of Wisconsin and from Handbook of Milk Composition, by R. G. Jensen, Academic Press, 1995.

Compiled  & Shared by- This paper is a compilation of groupwork provided by the

Team, LITD (Livestock Institute of Training & Development)

 Image-Courtesy-Google

 Reference-On Request.

MILK COMPOSITION & ITS CONSTITUENTS – NUTRITIONAL IMPORTANCE OF MILK

MILK COMPOSITION AND ITS CONSTITUENTS

MILK COMPOSITION & ITS CONSTITUENTS – NUTRITIONAL IMPORTANCE OF MILK

MILK COMPOSITION & ITS CONSTITUENTS – NUTRITIONAL IMPORTANCE OF MILK

IMPORTANCE OF MILK IN DIET

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